| A Quick and Efficient Technique for Detecting the Foreign DNAs and Their Expression in Large Transgenic Plant-Populations |
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| Miho AKIYOSHI, Kohki YOSHIDA and Noboru ENDO |
| It is generally required for the genetic manipulation to confirm whether introduced DNAs were effectively transformed into the plant genome and whether the foreign gene is fairly expressed. In order to select 100 or more individual plants containing target DNA or transcribed mRNA, quick and time-saving technique may play an important role. Because of simplicity, PCR technique has been used in many cases. One of the merits of PCR reaction is the less requirement of purification process of the DNAs and that relatively small amount of template DNA is sufficient. We tried to maximize sample size by modifying the experiment process in a short-cut way, particularly focusing on the process of the extraction, PCR or RT-PCR reaction, electrophoresis and the evaluation of target genes. As a result, it became possible to select individuals more than twice at one batch or in one half of conventional periods. Furthermore, the extraction and detection works of RNA became quick and less tedious. |
| keywords: |
Transgenics plant, PCR, Genomic PCR, RT-PCR |